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KMID : 1189220090360010045
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Korean Journal of Reproductive Medicine
2009 Volume.36 No. 1 p.45 ~ p.53
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Optimized Methods to Maintain Motility and Viability in Normozoospermic Males
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You Young-Ah
Oh Shin-Ae
Mohamed EA
Park Myung-Geol
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Abstract
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Objectives: To determinate the optimal culture condition to maintain lifespan in human sperm, we evaluated the effect of different temperature on sperm motility and viability up to 5 days in normal specimens.
Methods: Ejaculated semen samples with normal semen parameters were gently washed in HEPES buffered Tyrod’s-Albumin￢Lactate-Pyruvate (HTALP) media. Each 5 ml of HTALP + 0.3% albumin with 1×106 sperm/ml was incubated for 5 days in 37℃, 22℃, and 4℃. The sperm motility and kinematics were analyzed using computer assisted sperm analysis (CASA), membrane integrity was assessed by hypoosmotic swelling test (HOST), and capacitation status was evaluated by chlorotetracycline (CTC) fluorescence pattern. Each parameter was measured on day 1, 3, and 5, respectively.
Results: The motility, viability and live/uncapacitated pattern were demonstrated significantly in temperature- and time-dependent difference (p<0.05). While the sperm cultured for 1 day in each temperature was not significantly different, the sperm cell kept in 22℃ after 3 days were preserved sperm motility, viability, membrane integrity, and F pattern better than in other culture temperatures.
Conclusions: HTALP can be used a basic medium for culture and longevity preservation, and sperm cell kept at 22℃ is beneficial for assisted reproductive techniques.
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KEYWORD
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Optimal sperm culture condition, Longevity, Motility, Preservation
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